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An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose

Nature Chemical Biology DeLoache, W.C., Russ, Z.N., Narcross, L., Gonzales, A.M., Martin V.J.J., Dueber, J.E. Abstract Benzylisoquinoline alkaloids (BIAs) are a diverse family of plant-specialized metabolites that include the pharmaceuticals codeine and morphine and their derivatives. Microbial synthesis of BIAs

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A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly

ACS Synthetic Biology Lee, M.E., DeLoache, W.C., Cervantes, B., Dueber, J.E. Abstract Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of its long history in industrial fermentations. However, until recently, most synthetic biology systems have focused on

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Employing a combinatorial expression approach to characterize xylose utilization in Saccharomyces cerevisiae

 Metabolic Engineering Latimer, L.N., Lee, M.E., Medina-Cleghorn, D., Kohnz, R.A., Nomura, D.K., Dueber, J.E. Abstract Fermentation of xylose, a major constituent of lignocellulose, will be important for expanding sustainable biofuel production. We sought to better understand the effects of intrinsic

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Cell-free protein synthesis: Search for the happy middle

Biotechnology Journal Russ, Z.N., Dueber, J.E. Abstract Cell-free protein synthesis (CFPS) is a versatile technique gaining popularity because it allows researchers access to on-demand production of proteins. This Commentary by Zachary Russ and John Dueber discusses the latest research article

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Avoidance of Truncated Proteins from Unintended Ribosome Binding Sites within Heterologous Protein Coding Sequences

ACS Synthetic Biology Whitaker, W. R., Lee, H., Arkin, A. P., Dueber, J.E. Abstract Genetic sequences ported into non-native hosts for synthetic biology applications can gain unexpected properties. In this study, we exploredsequences functioning as ribosome binding sites (RBSs) within

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Identification and characterization of a galacturonic acid transporter from Neurospora crassa and its application for Saccharomyces cerevisiae fermentation processes

Biotechnology for Biofuels Benz JP1, Protzko RJ, Andrich JM, Bauer S, Dueber JE, Somerville CR. Abstract BACKGROUND: Pectin-rich agricultural wastes potentially represent favorable feedstocks for the sustainable production of alternative energy and bio-products. Their efficient utilization requires the conversion of

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