The Dueber Lab develops strategies for introducing designable, modular control over living cells. We are particularly interested in generating technologies for improving engineered metabolic pathway efficiency and directing flux. Our projects have applications in the development of biofuels, specialty chemicals, and environmentally friendly processes.
Latimer, L.N., Lee, M.E., Medina-Cleghorn, D., Kohnz, R.A., Nomura, D.K., Dueber, J.E.Metabolic Engineering. In press. 2014.
Xylose utilization in S. cerevisiae requires the introduction of three heterologous enzymes as well as high flux through the pentose phosphate pathway. To accomidate this increased flux and determine the optimal enzyme expression levels, we made a combinatorial ...
Whitaker, W. R., Lee, H., Arkin, A. P., & Dueber, J. E.ACS Synthetic Biology. 2014. doi:10.1021/sb500003x
Genetic sequences ported into non-native hosts for synthetic biology applications can gain unexpected properties. In this study, we explored sequences functioning as ribosome binding sites (RBSs) within protein coding DNA sequences (CDSs) that cause internal translation, resulting in ...
The 2015 Cold Spring Harbor Summer Course in Synthetic Biology has just announced UC Berkeley’s John Dueber as one of their upcoming instructors.Professor Dueber will be teaching a cutting-edge modular cloning (MoClo) Golden Gate strategy for rapidly synthesizing multi-gene devices. The focus will be on assembling devices for expression in Green Blue Jeans – Bakar Fellows Feature
Greener Blue Jeans, the Bakar Fellows Program’s February 2015 feature news piece, covers Dr. Dueber’s explorations into finding novel and cleaner ways to color blue jeans.