Blog Archives

Employing a biochemical protecting group for a sustainable indigo dyeing strategy

Nature Chemical Biology Hsu, T.M., Welner, D.H., Russ, Z.N., Cervantes, B., Prathuri, R.L., Adams, P.D., and Dueber, J.E. Abstract Indigo is an ancient dye uniquely capable of producing the signature tones in blue denim; however, the dyeing process requires chemical

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Bioproduction of a betalain color palette in Saccharomyces cerevisiae

Metabolic Engineering Grewal, P.S., Modavi, C., Russ, Z.N., Harris, N.C., and Dueber J.E. Abstract Betalains are a family of natural pigments found exclusively in the plant order Caryophyllales. All members of this chemical family are biosynthesized through the common intermediate

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Iterative optimization of xylose catabolism in Saccharomyces cerevisiae using combinatorial expression tuning

Biotechnology and Bioengineering Latimer, L. N., & Dueber, J.E. Abstract A common challenge in metabolic engineering is rapidly identifying rate-controlling enzymes in heterologous pathways for subsequent production improvement. We demonstrate a workflow to address this challenge and apply it to

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Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways

Nature Communications Deloache, W. C., Russ, Z. N., & Dueber, J. E. Abstract Compartmentalization of enzymes into organelles is a promising strategy for limiting metabolic crosstalk and improving pathway efficiency, but improved tools and design rules are needed to make

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Application of a Palladium-Catalyzed C-H Functionalization/Indolization Method to Syntheses of cis-Trikentrin A and Herbindole B.

Angewandte Chemie Leal, R.A. Bischof, C. Lee, Y.V., Sawano, S., McAtee, C.C., Latimer, L.N., Russ, Z.N, Dueber, J.E., Yu, J., Sarpong, R. We describe herein formal syntheses of the indole alkaloids cis-trikentrin A and herbindole B from a common meso-hydroquinone intermediate prepared by a

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A Barcoding Strategy Enabling Higher-Throughput Library Screening by Microscopy

ACS Synthetic Biology Chen, R., Rishi, H.S., Potapov, V., Yamada, M.R., Yeh, V.J., Chow, T., et al. Abstract Dramatic progress has been made in the design and build phases of the design-build-test cycle for engineering cells. However, the test phase

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An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose

Nature Chemical Biology DeLoache, W.C., Russ, Z.N., Narcross, L., Gonzales, A.M., Martin V.J.J., Dueber, J.E. Abstract Benzylisoquinoline alkaloids (BIAs) are a diverse family of plant-specialized metabolites that include the pharmaceuticals codeine and morphine and their derivatives. Microbial synthesis of BIAs

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A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly

ACS Synthetic Biology Lee, M.E., DeLoache, W.C., Cervantes, B., Dueber, J.E. Abstract Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of its long history in industrial fermentations. However, until recently, most synthetic biology systems have focused on

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Employing a combinatorial expression approach to characterize xylose utilization in Saccharomyces cerevisiae

 Metabolic Engineering Latimer, L.N., Lee, M.E., Medina-Cleghorn, D., Kohnz, R.A., Nomura, D.K., Dueber, J.E. Abstract Fermentation of xylose, a major constituent of lignocellulose, will be important for expanding sustainable biofuel production. We sought to better understand the effects of intrinsic

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Cell-free protein synthesis: Search for the happy middle

Biotechnology Journal Russ, Z.N., Dueber, J.E. Abstract Cell-free protein synthesis (CFPS) is a versatile technique gaining popularity because it allows researchers access to on-demand production of proteins. This Commentary by Zachary Russ and John Dueber discusses the latest research article

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